Published in Scientific Papers. Series D. Animal Science, Vol. LXVI, Issue 2
Written by Aurica Breica BOROZAN, Sorina POPESCU, Delia Gabriela DUMBRAVĂ, Diana RABA, Mirela Viorica POPA, Corina Dana MIȘCĂ, Daniela SCEDEI, Despina-Maria BORDEAN, Camelia MOLDOVAN
Even if the Romanian market has been overrun in recent years by non-indigenous hybrids, the high degree of adaptability of the Romanian varieties helps them to persist in the struggle for survival. The demands of organic consumption have determined a segment of the country's population to refocus on the consumption of indigenous apples or originating from other areas, but grown for a very long time in our country. The interest for these varieties with tasty fruits and special flavors led us to make a microbiological and molecular assessment. Molecular variability was investigated using ISSR (Inter Single Sequence Repeats) and SCoT (Start Codon Targeted) markers. The microbiological methodology is based on techniques for the isolation and determination of mesophilic bacteria, molds, yeasts, the presence of faecal bacteria and Escherichia coli, Salmonella and Shigella species. Escherichia coli was observed in some samples and enterococci were highlighted in only one sample. The results indicate a variation of the microbial groups in the varieties analyzed and the absence of species that can disturb serious the digestive tract (for example Salmonella and Shigella).
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