Published in Scientific Papers. Series D. Animal Science, Vol. LXVIII, Issue 1
Written by Milena BOZHILOVA-SAKOVA, Ivona DIMITROVA, Nevyana STANCHEVA
Growth differentiation factor 9 (GDF9) contains multiple mutations related to the fecundity in sheep. In the present experiment was studied the genetic variation in exon 1 (G1) and investigating of mutation c.260G>A, related to litter size in three sheep breeds raised in Bulgaria (Breznik, Bulgarian Dairy Synthetic Population and Black-headed Pleven). A total of 99 ewes were genotyped trough PCR-RFLP method. Results proved polymorphism in all of the three breeds. The highest genetic diversity was calculated in the BDSP population (0.385), where all three possible genotypes GG, AG and AA were identified with frequencies of 0.54, 0.41 and 0.05, respectively. The other two breeds are local breeds and as expected the genetic variation in them was lower. Genotypes GG and AG were found with very closed frequencies in both breeds. The statistical analysis manifested that all tested population were consisted with Hardy-Weinberg equilibrium.
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