Published in Scientific Papers. Series D. Animal Science, Vol. LXV, Issue 1
Written by Madalina Alexandra DAVIDESCU, Daniel SIMEANU, Cristina SIMEANU, Mihaela IVANCIA, Steofil CREANGA
This research aims to validate the most effective method of extracting DNA from a number of 20 blood samples collected from cows. Two methods were tested, namely: DNA extraction using a manual extraction kit-Promega and automatic DNA extraction, using the Maxwell 16 LEV Blood DNA kit-Promega. Following the quantification of DNA samples, by spectrophotometry technique, the best results were obtained by applying the automatic extraction method (77.37 ng/µl DNA concentration obtained by automatic extraction compared to 14.95 ng/µl DNA concentration obtained by manual extraction). Therefore, the effectiveness of this technique has been demonstrated, representing a first step in genomic analysis protocols. The accuracy of the subsequent results depends to a large extent on the results obtained by extracting the DNA from the samples. Therefore, the automatic DNA isolation method is recommended because it has a number of advantages: accuracy, reduced analysis time, low costs, reduced labor and ease of application. This technique can be successfully applied in the analysis of genetic diversity of different animal species.
[Read full article] [Citation]
